In this study, induction of regenerable callus from
mature embryos in eight Turkish barley varieties was analysed by using different
plant growth regulators (PGRs). Varying concentrations (0.5-4 mg l(-1)) of
2,4-dichlorophenoxyacetic acid (2,4-D) and dicamba (3,6-dichloro-o-anisic
acid) were tested for callus induction from mature embryos. Highest percent of
callus induction was observed in Bornova 92 variety (98.3%) on MS medium
supplemented with 4 mg l(-1) dicamba. Calli were transferred to regeneration
media with 0.5 mg l(-1) dicamba, 0.5 mg l(-1) zeatin riboside (ZR) and 2 mg
l(-1) thidiazuron (TDZ). Low concentrations of dicamba induced multiple shoots
during callus regeneration. When the effect of precultivation with 2,4-D or
dicamba on the shoot induction were evaluated, lower concentrations (< 4 mg
l(-1)) of auxins have been found optimal. On the regeneration medium with 0.5 mg
l(-1)
[url=http://www.pioneer-chem.com/productsclass.asp?BigClassName=Herbicide]dicamba[/url,
shoots were able to elongate up to 20 cm and shoot numbers were between 1-23 per
callus. The use of ZR led to formation of short shoot buds and somatic embryos
in 2 weeks period. The effect of TDZ was different from other PGRs by inducing
green solid sectors on calli surfaces (Total 51 sectors/20 callus/Akhisar
variety). Five plantlets have been grown from these solid cell clumps and
transferred to specific media for root formation. As a result, five varieties
(Suleyman Bey, Bornova 92, Vamyk Hoca, Kaya and Akhisar) tested in our study
showed the potential to produce regenerable callus by using low amounts of
dicamba or TDZ. The optimization process starts from culturing embryos to
plantlet formation took nearly 4 weeks.]
没有评论:
发表评论