Ten barley elite lines and cultivars were studied
to determine the influence of genotypes, different seed cutting, media and plant
hormones on the embryo-derived callus induction and plant regeneration. The
results indicated that the callus induction rate of seed cutting longitudinally
was higher than that of seed cutting laterally. Modified MS improved callus
formation. Barley cultivars showed different response to plant hormones 2,4-D
and Dicamba. The induced primary callus ultimately yielded two types of
embryonic callus after subculture of 3 cycles. Regeneration media added
different concentration of organic nutrients influenced plant regeneration of
different barley genotypes. Albinos were observed in several cultivars after
long-time subculture. Callus could be induced from all the tested cultivars,
however, plant regeneration was only obtained in several genotypes. Among the
ten barley cultivars 87-3175,87-0053,97-4010,97-6004 and 208813-509 were
identified with good performance in callus induction and plant regeneration.
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