By: H. F Lin, J. W Huang, T. F Hsieh
In the study, Naito’s soil-cover culture method was modified for production of hymenia of Thanatephorus cucumeris, the causal agent of Chinese amaranth foliage blight. Result showed that the peat moss-soil-cover culture method (PSC method) was more effective in producing hymenia of both T. cucumeris RSA-03 and RSA-09 isolates. The procedures of PSC method were as follows: (l) to inoculate each Rhizoctonia isolate onto potato-yeast extract-dextrose agar plate in a 9-cm petri dish, (2) to incubate the fungus at 28℃ for 4 days until its colony completely covered the agar plate surface, (3) to cover the agar plate surface with 90ml soil [included 40% (v/v) BVB No.4 peat moss and maintained the soil moisture at 40-50% (v/v)], (4) to keep the soil-cover plates in moist chamber. Hymenial formation was observed after incubation for 4 days. The PSC method was suitable for hymenial formation of the pathogen and enable to enhance markedly 3-4 fold amount of hymenial production as compared to Naito’s soil-cover-culture method. The factors affecting hymenial formation of the pathogen included temperature, humidity, light, aeration, and culture substrate. The suitable temperatures and pH values of covered soil for R. solani RSA03 and RSA-09 hymenial formation were the range of 24 – 28℃ and pH 5 – 7, respectively. Covered soils amended with various organic matters and fungicides did significantly influence the hymenia formation of T. cucumeris RSA-03 and RSA-09. Result showed that the hymenia of the fungus were completely inhibited by I %(v/v) fish meal, tea seed pomace and chinaberry meal. All the tested fungicides, such as mancozeb, benomyl, carbendazim, flutolanil, PCNB, iprodione and pencycuron were significantly effective in inhibiting the hymenial formation.
In the study, Naito’s soil-cover culture method was modified for production of hymenia of Thanatephorus cucumeris, the causal agent of Chinese amaranth foliage blight. Result showed that the peat moss-soil-cover culture method (PSC method) was more effective in producing hymenia of both T. cucumeris RSA-03 and RSA-09 isolates. The procedures of PSC method were as follows: (l) to inoculate each Rhizoctonia isolate onto potato-yeast extract-dextrose agar plate in a 9-cm petri dish, (2) to incubate the fungus at 28℃ for 4 days until its colony completely covered the agar plate surface, (3) to cover the agar plate surface with 90ml soil [included 40% (v/v) BVB No.4 peat moss and maintained the soil moisture at 40-50% (v/v)], (4) to keep the soil-cover plates in moist chamber. Hymenial formation was observed after incubation for 4 days. The PSC method was suitable for hymenial formation of the pathogen and enable to enhance markedly 3-4 fold amount of hymenial production as compared to Naito’s soil-cover-culture method. The factors affecting hymenial formation of the pathogen included temperature, humidity, light, aeration, and culture substrate. The suitable temperatures and pH values of covered soil for R. solani RSA03 and RSA-09 hymenial formation were the range of 24 – 28℃ and pH 5 – 7, respectively. Covered soils amended with various organic matters and fungicides did significantly influence the hymenia formation of T. cucumeris RSA-03 and RSA-09. Result showed that the hymenia of the fungus were completely inhibited by I %(v/v) fish meal, tea seed pomace and chinaberry meal. All the tested fungicides, such as mancozeb, benomyl, carbendazim, flutolanil, PCNB, iprodione and pencycuron were significantly effective in inhibiting the hymenial formation.
没有评论:
发表评论