By: Nirmal Kumar, J.I. , Rita N Kumar, Manmeet Kaur Amb, Anubhuti Bora
The biotransformation of four pesticides to different functional groups on four days interval was studied using biomass of three different cyanobacterial species-Anabaena fertilissima Rao, Aulosira fertilissima Ghose and Westiellopsis prolifica Janet by Fourier Transform Infrared (FT-IR) Spectroscopy. This study demonstrated different absorption peaks associated with phenols and alcohols, aromatic amines, aromatics, carboxylic acids, 1°, 2° amines, alkynes, alkyl halides and nitro compounds generated by different concentration treatments of pesticides on Anabaena fertilissima. In 2,4-D and pencycuron treated Aulosira fertilissima the peaks of nitro compounds , 1°, 2° amines, aromatics, aromatic amines, carboxylic acids, 1°amines and aliphatic amines were involved in pesticide-biomass interaction. Moreover, the IR bands of Tebuconazole treated Aulosira fertilissima suggested unique functionalities, i.e., 1°, 2° amines and 1°amines, whereas no new peaks were recorded in Endosulfan treatments as compared to the untreated cultures. With respect to untreated biomass of Westiellopsis prolifica, differences in the peak patterns of four selected pesticide treated cultures were attributable to the presence of aromatic amines, aliphatic amines, carboxylic acids, aromatics, alkenes and 1°amines. Several other such changes were also evident and revealed that FT-IR technique proves to be an efficient tool for detecting structural and compositional changes in functional groups and probable binding sites induced by the presence of a pesticide contamination.
The biotransformation of four pesticides to different functional groups on four days interval was studied using biomass of three different cyanobacterial species-Anabaena fertilissima Rao, Aulosira fertilissima Ghose and Westiellopsis prolifica Janet by Fourier Transform Infrared (FT-IR) Spectroscopy. This study demonstrated different absorption peaks associated with phenols and alcohols, aromatic amines, aromatics, carboxylic acids, 1°, 2° amines, alkynes, alkyl halides and nitro compounds generated by different concentration treatments of pesticides on Anabaena fertilissima. In 2,4-D and pencycuron treated Aulosira fertilissima the peaks of nitro compounds , 1°, 2° amines, aromatics, aromatic amines, carboxylic acids, 1°amines and aliphatic amines were involved in pesticide-biomass interaction. Moreover, the IR bands of Tebuconazole treated Aulosira fertilissima suggested unique functionalities, i.e., 1°, 2° amines and 1°amines, whereas no new peaks were recorded in Endosulfan treatments as compared to the untreated cultures. With respect to untreated biomass of Westiellopsis prolifica, differences in the peak patterns of four selected pesticide treated cultures were attributable to the presence of aromatic amines, aliphatic amines, carboxylic acids, aromatics, alkenes and 1°amines. Several other such changes were also evident and revealed that FT-IR technique proves to be an efficient tool for detecting structural and compositional changes in functional groups and probable binding sites induced by the presence of a pesticide contamination.
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