Huang, Yu-Ju
The effect of fungicides carbendazim, pencycuron and insecticide imidacloprid on soil bacterial community structure was studied. The WaiPu sandy loam soil used in this experiment was sampled from strawberry field locate in Da-Hu of Mia Lei County. Degradation rate of pesticides were determined by the residues of pesticides in soil which sampled at different intervals. The variation of bacterial communities during incubation was performed with counting the 16S rDNA band on denaturing gradient gel electrophoresis patterns.
From the result, the half-life of 10 mg Kg-1 carbendazim in soil incubated at 25 and 40 ℃ was 105 and 103 days, respectively. And there were no degradation found in a concentration 100 mg Kg-1 at the same incubated temperature. It showed very slow dissipation rate than the other two pesticides in the same treatment. Meanwhile, all three pesticides showed slower dissipation rate in sterile soil than in no sterile soil. It means microbes involved in the dissipation of these three pesticides in soil. The DGGE fingerprint showed that some soil bacterial communities would be inhibited, some would be introduced and some would not be affected by treated with pesticides carbendazim, pencycuron or imidacloprid. Besides, the DGGE fingerprint also showed that the soil bacterial community structure would be different at different incubation temperature.
The effect of fungicides carbendazim, pencycuron and insecticide imidacloprid on soil bacterial community structure was studied. The WaiPu sandy loam soil used in this experiment was sampled from strawberry field locate in Da-Hu of Mia Lei County. Degradation rate of pesticides were determined by the residues of pesticides in soil which sampled at different intervals. The variation of bacterial communities during incubation was performed with counting the 16S rDNA band on denaturing gradient gel electrophoresis patterns.
From the result, the half-life of 10 mg Kg-1 carbendazim in soil incubated at 25 and 40 ℃ was 105 and 103 days, respectively. And there were no degradation found in a concentration 100 mg Kg-1 at the same incubated temperature. It showed very slow dissipation rate than the other two pesticides in the same treatment. Meanwhile, all three pesticides showed slower dissipation rate in sterile soil than in no sterile soil. It means microbes involved in the dissipation of these three pesticides in soil. The DGGE fingerprint showed that some soil bacterial communities would be inhibited, some would be introduced and some would not be affected by treated with pesticides carbendazim, pencycuron or imidacloprid. Besides, the DGGE fingerprint also showed that the soil bacterial community structure would be different at different incubation temperature.
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