BY:Vanessa Andaló; Alcides Moino Jr.; Lenira V.C.
Santa-Cecília; Giselle C. Souza
Several chemical substances are used to control insects, diseases and weeds,
however many of these products are toxic to mankind and the animals, besides
reducing the potential of pest control by predators, parasitoids and pathogens.
The integrated control using selective chemical pesticides and entomopathogenic
fungi is a viable strategy, however some of these products can impact these
microorganisms, reducing vegetative growth, viability and sporulation. The
objectives of this work were to evaluate the effect of chemical pesticides used
in the coffee crop on the entomopatogenic fungus Beauveria bassiana (isolate UEL
114), for the control of the cofee root mealybug. A fungal suspension of 1 x 107
viable conidia/ml was added to solutions of the products at the recommended
concentrations. After 1h, conidia were inoculated onto PDA medium, and
quantification of germinated conidia assessed after 20h. The vegetative growth
and sporulation were appraised eight days after the fungus inoculation onto PDA
medium containing the products at recommended concentrations and maintained at
the temperature of 25 ± 1ºC, 12h photofase and 70 ± 10% relative
humidity. The mean diameter of the colonies was measured and conidial production
quantified in a Neubauer chamber. Azafenidyne, Quintozene, Symazine + Ametryne,
2,4-D, Acetochlor and Oxyfluorfen affected the conidial germination.
Thiamethoxan, Imidacloprid, Carbofuran and Pencycuron were compatible;
whereas Glyphosate, Dimetilurea, Azafenidine, Quintozene, Symazine + Ametryne,
2,4-D, Acetochlor and Oxyfluorfen significantly impacted vegetative growth and
sporulation of B. bassiana UEL 114.Yangzhou pioneer chemical CO.,LTD.
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