The present study was undertaken to evaluate
genotoxic potential of two auxinic herbicides [2,4-dicholorophenoxy acetic acid
(2,4-D) and 3,6-dichloro-2-methoxybenzoic acid (Dicamba)] in the roots of common
bean (Phaseolus vulgaris L.) seedlings. Two-day-old etiolated seedlings were
treated with 10 ppm methyl methanesulfonate (MMS, positive control) or 0.1, 0.2,
or 0.3 ppm of either 2,4-D or Dicamba. At the end of a 96 h growth period, root
growth, total soluble protein content, DNA damage in individual cells (comet
assay scores) and randomly amplified polymorphic DNA (RAPD) profiles were used
as endpoints of genotoxicity. 2,4-D and Dicamba were clearly
dose-dependent root growth inhibitors. Total soluble protein content was
significantly decreased in the positive control and at high concentrations (0.2
and 0.3 ppm) of Dicamba. Soluble protein content increased significantly only at
0.3 ppm 2,4-D (P<0.05). In the comet assay, DNA fragmentation increased in a
dose-dependent manner. The diagnostic and phenetic analyzes of appeared and/or
disappeared RAPD bands indicated that dose-dependent DNA polymorphism was
induced by both herbicides. Genomic template stability was significantly
affected at all 2,4-D and Dicamba doses tested. Overall 2,4-D and Dicamba have similar
effects on DNA damage detected by comet and RAPD assays.
Yangzhou pioneer chemical CO.,LTD
没有评论:
发表评论