The secondary effects of pencycuron on cell membrane of Rhizoctonia solani AG4 were investigated by the observation of giant protoplast formation and lipid per¬oxidaiton. Compared to protoplasts initially produced from the strains, protoplasts of R. solani R-C (sensitive strain) and Rh-131 (non-sensitive strain) increased in their size by 2.0-3.5 times 12 h after incubation in potato-dextrose broth containing novozyme (7 mg/ml) and β-glucuronidase (60 µl/ml) with 0.6 M mannitol (pH 5.2). The increase of protoplast size in R-C was slightly inhibited from 13.8 µm without pencycuron to 10.3 µm with 1.0 µg/ml of pencycuron. However, the size of giant protoplast of Rh-131 was not affected by the pencycuron treatment. Both strains R-C and Rh-131 did not exhibit the lipid peroxidation 12 h after the application of 1.0 µg/ml pencycuron. The remarkable peroxidation of membrane lipid was observed only in R-C 24 h after pencycuron application, but not in Rh¬131. Although the inhibition of giant protoplast forma¬tion and the membrane lipid peroxidation were observed only in the sensitive strain R-C by pencycuron, it is diffi¬cult to conclude that these are the primary mechanism of pencycuron. The mild activity of pencycuron on the inhibition of giant protoplast formation and late mem¬brane lipid peroxidation in the fungicide-sensitive strain did not coincide with the dramatic activity of pencycu¬ron in R. solani. Therefore, our results suggest that inhi¬bition of giant protoplast formation and membrane lipid peroxidation is the secondary effect of pencycuron.
Yangzhou Pioneer Chemical Co., Ltd , which is the subsidiary of Pioneer International Industry Limited.It was founded in 2005,with the authority of independent import and export. The head office has five subsidiaries:Yangzhou Pioneer Chemical Co.,Ltd., Lianshui Pioneer Chemical Co., Ltd(preparation facory),Changzhou Agro-surfactant Co.,Ltd(surfactant factory),Yangzhou Lianfa Science and Technology Co.,Ltd(test service center)and Yangzhou Huijie Imp&Exp company.
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