显示标签为“Dicamba”的博文。显示所有博文
显示标签为“Dicamba”的博文。显示所有博文

2012年7月3日星期二

Soil persistence studies with [14C]dicamba, propanil and bromoxynil in herbicidal mixtures



By: Axel Hengefeld and Reinhard Nast
The persistence of bromoxynil (3,5-dibromo-4-hydroxybenzonitrile), [14C]dicamba (3,6-dichloro-2-methoxybenzoic-7-14C acid) and propanil [N-(3,4-dichlorophenyl)propionamide] at rates equivalent to 1 kg ha−1, were studied under Laboratory conditions in a clay loam, a heavy clay and a sandy loam at 85% of field capacity and at 20±1 degree, both singly and in the presence of herbicides normally applied with these chemicals as tank-mix or split-mix components.


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2012年7月2日星期一

Dicamba, stress, and distorted soybean leaves


by Bob Hartzler, Department of Agronomy
A fairly common occurrence in late June and July is the appearance of distorted leaves on soybean plants. The most common cause of this response is exposure to a growth regulator herbicide (2,4-D; dicamba; etc.). Dicamba is the most commonly used growth regulator herbicide in Iowa crop production, and is present in numerous products (Banvel, Clarity, Marksman, Distinct, Status, and many others).
Soybeans may be exposed to dicamba in three ways: spray particle drift, vapor drift (volatilization), and sprayer contamination. Injury from spray drift or sprayer contamination usually is fairly straightforward in diagnosing, but questions often arise regarding volatilization. Volatilization occurs when dicamba is sprayed, lands on the target site, but later evaporates and moves from the treated field. The potential for volatilization is affected by many factors. As temperatures exceed 85 °F, the threat of volatilization increases. 
Dicamba is more likely to evaporate off corn leaves than soil, so the risk increases with late applications of dicamba to corn since more product will be intercepted by corn leaves. Risk of volatilization varies among dicamba formulations, with the dimethlyamine salt (Banvel) greater than the diglycolamine salt (Clarity) or sodium salt (Distinct, Status). Studies have shown that the majority of volatilization occurs within 2-3 days of application. Determining the potential for yield impact from growth regulator herbicides shortly after exposure is difficult, but if only two or three leaves show slight distortion, it is likely yields will not be affected. Studies found that if soybean height at the end of the season was not affected, then yields usually were not affected by dicamba exposure.It generally is accepted that occasionally soybeans will develop symptoms similar to that of growth regulator herbicides in the absence of the herbicide. These symptoms typically occur during stress or periods of rapid fluctuating weather. Under these conditions, the symptoms typically appear uniformly across a field, rather than displaying a spray or drift pattern. With this type of response, the cupping is relatively minor and limited to one or two leaves.


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2012年6月27日星期三

About dicamba: Cancer incidence among pesticide applicators exposed to dicamba in the agricultural health study


BACKGROUND:
Dicamba is an herbicide commonly applied to crops in the United States and abroad. We evaluated cancer incidence among pesticide applicators exposed to dicamba in the Agricultural Health Study, a prospective cohort of licensed pesticide applicators in North Carolina and Iowa.
METHODS:
Detailed pesticide exposure information was obtained through a self-administered questionnaire completed from 1993 to 1997. Cancer incidence was followed through 31 December 2002 by linkage to state cancer registries. We used Poisson regression to estimate rate ratios and 95% confidence intervals for cancer subtypes by tertiles of dicamba exposure. Two dicamba exposure metrics were used: lifetime exposure days and intensity-weighted lifetime exposure days (lifetime days x intensity score).
RESULTS:
A total of 41,969 applicators were included in the analysis, and 22,036 (52.5%) reported ever using dicamba. Exposure was not associated with overall cancer incidence nor were there strong associations with any specific type of cancer. When the reference group comprised low-exposed applicators, we observed a positive trend in risk between lifetime exposure days and lung cancer (p = 0.02), but none of the individual point estimates was significantly elevated. We also observed significant trends of increasing risk for colon cancer for both lifetime exposure days and intensity-weighted lifetime days, although these results are largely due to elevated risk at the highest exposure level. There was no apparent risk for non-Hodgkin lymphoma.
CONCLUSIONS:
Although associations between exposure and lung and colon cancer were observed, we did not find clear evidence for an association between dicamba exposure and cancer risk.


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2012年6月24日星期日

Dicamba monooxygenase: structural insights into a dynamic Rieske oxygenase that catalyzes an exocyclic monooxygenation


Dicamba (2-methoxy-3,6-dichlorobenzoic acid) O-demethylase (DMO) is the terminal Rieske oxygenase of a three-component system that includes a ferredoxin and a reductase. It catalyzes the NADH-dependent oxidative demethylation of the broad leaf herbicide dicamba. DMO represents the first crystal structure of a Rieske non-heme iron oxygenase that performs an exocyclic monooxygenation, incorporating O(2) into a side-chain moiety and not a ring system. The structure reveals a 3-fold symmetric trimer (alpha(3)) in the crystallographic asymmetric unit with similar arrangement of neighboring inter-subunit Rieske domain and non-heme iron site enabling electron transport consistent with other structurally characterized Rieske oxygenases. While the Rieske domain is similar, differences are observed in the catalytic domain, which is smaller in sequence length than those described previously, yet possessing an active-site cavity of larger volume when compared to oxygenases with larger substrates. Consistent with the amphipathic substrate, the active site is designed to interact with both the carboxylate and aromatic ring with both key polar and hydrophobic interactions observed. DMO structures were solved with and without substrate (dicamba), product (3,6-dichlorosalicylic acid), and either cobalt or iron in the non-heme iron site. The substitution of cobalt for iron revealed an uncommon mode of non-heme iron binding trapped by the non-catalytic Co(2+), which, we postulate, may be transiently present in the native enzyme during the catalytic cycle. Thus, we present four DMO structures with resolutions ranging from 1.95 to 2.2 A, which, in sum, provide a snapshot of a dynamic enzyme where metal binding and substrate binding are coupled to observed structural changes in the non-heme iron and catalytic sites. 


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2012年6月20日星期三

Comparison of 2,4-D and dicamba treatments for embryo development and plant regeneration in crosses between Lolium multiflorum Lam. and Dactylis glomerata L.


BY:Toshinori Komatsu, Kazuhiro Uchiyama, Akira Arakawa, Kazuhiro Tase
We compared the effects of 2,4-dichlorophenoxy acetic acid (2,4-D) and 6-dichloro-2-methoxybenzoic acid (dicamba) treatments on embryo development and plant regeneration in crosses between Lolium multiflorum Lam. and Dactylis glomerata L. Two embryos (1.1% of pollinated florets) were obtained after 2,4-D treatment for 2 days. They germinated on hormone-free Murashige and Skoog (MS) medium containing 1 g L−1 yeast extract, 70 g L−1 sucrose and 6 g L−1 agar, and developed into green seedlings. dicamba treatment for 2 days gave higher frequencies of embryo development (4.3% of pollinated florets) than did 2,4-D treatment, but only one embryo (0.4% of pollinated florets) germinated on hormone-free MS agar medium, and no seedling was obtained. These results show that 2,4-D is more effective than dicamba at producing F1 hybrids on hormone-free MS agar medium. The F1 plant was intermediate between the parents in height, inflorescence morphology and lower leaf appearance. The folding of leaves in young shoots and the number of glumes resembled those of D. glomerata. Anthers did not dehisce. The F1 plant was an aneuploid with 26 chromosomes. The mean chromosome association was 0.04 trivalents, 10.36 bivalents and 5.16 univalents at metaphase I in pollen mother cells.
 


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2012年6月19日星期二

Dicamba and Sugar Effects on CallusInduction and Plant Regeneration from Mature Embryo Culture of Wheat


BY:Jiang-ping REN, Ph D, Xin-guo WANG, Jun YIN
To establish a highly efficient plantregeneration system for wheat genetic transformation, the effects of three different concentrations of dicamba and two different sugar types on callusinduction and plantregeneration from matureembryocultures were evaluated. Callusinduction and plantregeneration were obtained from matureembryos of two commercial cultivars Zhoumai 18 and Yumai 34 (Triticum aestivum L.) cultured on L3 basal medium. The results showed that the efficiency of matureembryoculture was significantly influenced by the genotypes, sugar types and dicamba concentrations. 4 mg L−1dicamba proved the best effective for inducing embryogenic callus and also gave the highest proportion of plants regenerated across the two cultivars. Substitution of maltose by sucrose significantly improved the plantregeneration efficiency in both cultivars. There was a significant interaction between genotype-by-sugar types, and sugar types-bydicamba concentrations. Overall, Zhoumai 18 gave the highest frequency of plantregeneration (82.65%) when dicamba concentration was 4.0 mg L−1 and with sucrose in initial callusinduction. These results will facilitate genetic transformation work with elite wheat.


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2012年6月17日星期日

About dicamba: dmo methods and compositions


The invention provides for identification and use of crystal structures of Dicamba monooxygenase (DMO) that may be complexed with iron or cobalt cofactor and substrate (dicamba), or product (DCSA) in order to define residues important for enzymatic structure and function. Methods of using such structures are described. Data storage media comprising the crystal structural coordinate information are also described. 



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2012年6月14日星期四

About dicamba:utilized a combination of nitrogen fertilizers rates and herbicide treatments


reported that all herbicide treatments were effective on both thistles with or without fertilizer. They indicated that fertilized plots had a greater thistle biomass than the unfertilized check in the three-year experiment. They further reported that ammonium nitrate with either clopyralid in 1978, clopyralid + 2, 4-D in 1979 and dicamba + 2, 4-D in 1980 reduced grass biomass compared to non-fertilized plots; however, there was no determination that biomass was reduced due to herbicide injury, which was observed in some treatments in 1978 (Reece and Wilson 1983). Donald (1993) studied the effectiveness of long-term herbicide treatments applied in late September for the control of Canada thistle shoot density on non-cropped, untilled abandoned farmland. Clopyralid, glyphosate and picloram were effective in reducing Canada thistle at certain high rates in the first two years of the trial. The fall-applied treatments of clopyralid and picloram took fewer years of treatments to reduce shoot density than did glyphosate or dicamba. Clopyralid at 840 g/ha or picloram at 560 g/ha applied in the fall for three consecutive years inhibited, or almost prevented, shoot emergence the third growing season, while glyphosate and dicamba did not reduce shoot emergence. Miller and Lym (1998) conducted several field experiments on the control of Canada thistle with herbicides and cultivation treatments in corn and soybeans. Photoperiod determinations of Canada thistle and clopyralid absorption and translocation at different stages of growth were evaluated. The herbicide and cultivation treatments resulted in clopyralid and bentazon having 42% and 44% control 4 MAT in corn and soybeans, respectively.  


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2012年6月12日星期二

About dicamba:Scientists Develop Broadleaf Crops That Resist Dicamba Herbicide


University of Nebraska scientists have developed a way to give broadleaf crops like soybeans the ability to resist the herbicide dicamba. According to the EPA, dicamba is a selective benzoic acid herbicide registered for the control of certain broadleaf weeds and woody plants. The chemical kills plants by causing rapid, uncontrolled cell growth.
The Bureau of National Affairs reports that the scientists say that dicamba-based herbicides, sold under trade names such as Banvil and Clarity, are relatively inexpensive and environmentally benign because they disappear quickly in plants and soil. However, they said that dicamba also kills broadleaf crops. As a result, its use has been limited to corn and other grassy crops. 



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2012年6月8日星期五

About dicamba:Induction and screening of embryonic callus from immature embryo culture of maize inbred line


BY: LI Hai-xia,QIN Wen-juan,TANG Ji-hua,LIU Zong-hua,CHANG Ji-ping,HU Yan-min
Effect of 2,4-D and dicamba on the induction and subculture of callus from maize inbred line was compared with Zheng 58,Zheng 22 and Ye 478 as experimental materials.The results show that the induction effect of dicamba on the calli from the immature embryos of 3 maize inbred lines was better than that of the 2,4-D;the subculture medium with 2 mg L-1 2,4-D adapted to 3 types of genotypes;Zheng 22,more easily induced high quality callus,can be induced directly on induction medium containing 2 mg L-1 2,4-D,then can be cultured on subculture medium containing 2 mg L-1 2,4-D;as for the materials induced callus more difficultly,such as Zheng 58 and Ye 478,they should be induced on induction medium containing 2~3 mg L-1 dicamba,and should be cultured on subculture medium containing 2 mg L-1 2,4-D.This method was conducive to obtain embryonic callus.


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2012年6月6日星期三

About dicamba: Methods and materials for making and using transgenic dicamba-degrading organisms


BY: Weeks, Donald P. Wang, Xiao-zhuo  Herman, Patricia L.
The invention provides isolated and at least partially-purified dicamba-degrading enzymes, isolated DNA molecules coding for dicamba-degrading enzymes, DNA constructs coding for dicamba-degrading enzymes, transgenic host cells comprising DNA coding for dicamba-degrading enzymes, and transgenic plants and plant parts comprising one or more cells comprising DNA coding for dicamba-degrading enzymes. Expression of the dicamba-degrading enzymes results in the production of dicamba-degrading organisms, including dicamba-tolerant plants. The invention further provides a method of controlling weeds in a field containing the transgenic dicamba-tolerant plants of the invention and a method of decontaminating a material containing dicamba comprising applying an effective amount of a transgenic microorganism or dicamba-degrading enzyme(s) of the invention to the material. Finally, the invention provides a method of selecting transformed plants and plant cells based on dicamba tolerance and a method of selecting or screening transformed host cells, intact organisms and parts of organisms based on the fluorescence of 3,6-dichlorosalicylic acid produced as a result of dicamba degradation


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2012年5月30日星期三

About dicamba :Mutant analysis in Arabidopsis provides insight into the molecular mode of action of the auxinic herbicide dicamba


BY: Gleason C, Foley RC, Singh KB.
Herbicides that mimic the natural auxin indole-3-acetic acid are widely used in weed control. One common auxin-like herbicide is dicamba, but despite its wide use, plant gene responses to dicamba have never been extensively studied. To further understand dicamba"s mode of action, we utilized Arabidopsis auxin-insensitive mutants and compared their sensitivity to dicamba and the widely-studied auxinic herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). The mutant axr4-2, which has disrupted auxin transport into cells, was resistant to 2,4-D but susceptible to dicamba. By comparing dicamba resistance in auxin signalling F-box receptor mutants (tir1-1, afb1, afb2, afb3, and afb5), only tir1-1 and afb5 were resistant to dicamba, and this resistance was additive in the double tir1-1/afb5 mutant. Interestingly, tir1-1 but not afb5 was resistant to 2,4-D. Whole genome analysis of dicamba-induced gene expression showed that 10 hours after application, dicamba stimulated many stress-responsive and signalling genes, including those involved in biosynthesis or signalling of auxin, ethylene, and abscisic acid (ABA), with TIR1 and AFB5 required for the dicamba-responsiveness of some genes. Research into dicamba-regulated gene expression and the selectivity of auxin receptors has provided molecular insight into dicamba-regulated signalling and could help in the development of novel herbicide resistance in crop plants.


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2012年5月29日星期二

About dicamba : Regeneration capacity of mature embryo-derived callus in barley (hordeum vulgare l.)


In this study, induction of regenerable callus from mature embryos in eight Turkish barley varieties was analysed by using different plant growth regulators (PGRs). Varying concentrations (0.5-4 mg l(-1)) of 2,4-dichlorophenoxyacetic acid (2,4-D) and dicamba (3,6-dichloro-o-anisic acid) were tested for callus induction from mature embryos. Highest percent of callus induction was observed in Bornova 92 variety (98.3%) on MS medium supplemented with 4 mg l(-1) dicamba. Calli were transferred to regeneration media with 0.5 mg l(-1) dicamba, 0.5 mg l(-1) zeatin riboside (ZR) and 2 mg l(-1) thidiazuron (TDZ). Low concentrations of dicamba induced multiple shoots during callus regeneration. When the effect of precultivation with 2,4-D or dicamba on the shoot induction were evaluated, lower concentrations (< 4 mg l(-1)) of auxins have been found optimal. On the regeneration medium with 0.5 mg l(-1) [url=http://www.pioneer-chem.com/productsclass.asp?BigClassName=Herbicide]dicamba[/url, shoots were able to elongate up to 20 cm and shoot numbers were between 1-23 per callus. The use of ZR led to formation of short shoot buds and somatic embryos in 2 weeks period. The effect of TDZ was different from other PGRs by inducing green solid sectors on calli surfaces (Total 51 sectors/20 callus/Akhisar variety). Five plantlets have been grown from these solid cell clumps and transferred to specific media for root formation. As a result, five varieties (Suleyman Bey, Bornova 92, Vamyk Hoca, Kaya and Akhisar) tested in our study showed the potential to produce regenerable callus by using low amounts of dicamba or TDZ. The optimization process starts from culturing embryos to plantlet formation took nearly 4 weeks.] 


2012年5月28日星期一

About dicamba: Response of Strip-tilled Cotton to Preplant Applications of Dicamba and 2,4-D


BY: Alan C. York, A. Stanley Culpepper, and Alexander M. Stewart
Conservation tillage is being adopted by cotton(Gossypium hirsutum L.) growers across the southeastern United States. Glyphosate is commonly applied prior to planting to control winter vegetation, but preplant control of certain weeds, especially cutleaf eveningprimrose (Oenothera laciniata Hill), requires 2,4-D or dicamba mixed with glyphosate. A field experiment was conducted at seven locations to determine response of strip-tilled cotton to dicamba diglycolamine salt at 280 and 560 g acid equivalent (a.e.) ha-1 or 2,4-D dimethylamine salt at 530 and 1060 g a.e. ha-1 applied 1 to 6 wk before planting (WBP). These rates are 1 and 2 times the labeled rates. No adverse effects on cotton were noted when 2,4-D was applied 3 or more WBP. Visible leaf distortion on more than 10% of the seedlings and stand reduction was noted at 1 of 7 locations when 2,4-D was applied 2 WBP and at 2 of 7 locations when applied 1 WBP. Cotton yield was not reduced by 2,4-D at 530 g ha-1 at any application time, and it was reduced by 2,4-D at 1060 g ha-1 applied 1 WBP at 1 of 7 locations. Dicamba at 280 g ha-1 applied 3 or more WBP did not cause leaf distortion or affect stands. Leaf distortion on more than 10% of seedlings was noted at 1 of 7 locations with 280 g ha-1 dicamba applied 2 WBP, but yield was unaffected regardless of time of application. Dicamba at 560 g ha-1 applied 3 WBP caused leaf distortion on more than 10% of the seedlings and reduced yield at 1 of 7 locations. Cotton response to dicamba, but not 2,4-D, was generally correlated with rainfall between application and planting.



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2012年5月25日星期五

2, 4-D and Dicamba as a Harvest Aid


If broadleaf weeds are going to interfere with harvest, 2,4-D can be applied at 0.75 to 1.5 lbs/A (1.5 to 2 pts/A of a 4 lb/gal a.i. product) at the dough stage of spring wheat, barley or rye. Not all 2,4-D formulations are labeled for preharvest applications.
Some 2,4-D labels only allow use on wheat, others allow use on wheat and barley and others allow use on wheat, barley and rye. Choose a brand that is labeled for use on the intended small grain crop. An ester formulation will give better control and quicker burndown than amine formulation. If using an ester formulation, use a low volatile formulation to reduce vapor drift potential. If using an amine, at least 2 pts/A is needed for larger weeds. Do not expect good control on large pigweed or kochia or wild buckwheat. Large kochia and other weeds with large stems may not burn down and may stay green for an extended period.
2,4-D can be tank mixed with Glyphosate on spring wheat and durum for additional broadleaf control and grass control. See the following paragraph for restrictions and read the label.
The labels of most formulations of 2,4-D have a grazing restriction of no dairy and 7 days for meat animals and a 30 day have restriction. Do not feed straw to livestock.
Apply Dicamba at 0.5 pt/A + 2,4-D at 1 to 2 pt/A when wheat is in the hard dough stage and the green color is gone from the nodes of the stem. Dicamba will provide additional control of wild buckwheat, kochia, common lambsquarters, pigweed spp., sunflower, and Russian thistle.

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2012年5月22日星期二

A report about pioneer chemical’s dicamba


Dicamba (3,6-dichloro-2-methoxybenzoic acid) has proven to be a highly beneficial herbicide. It is normally applied as the dimethylamine salt. Unfortunately, this salt has a volatility that causes the product, under certain conditions of application, to come into contact with desirable plants, such as soybeans, that it can damage. This is caused by its volatility.
It is therefore an object of the present invention to find a derivative of dicamba that will maintain or improve the beneficial properties of the product while lowering the volatility.
It is another object of the present invention to locate a compound that has the herbicidal properties of dicamba with enhanced solubility characteristics.
Other objects of the present invention will be seen from the ensuing description.
It has been found that the 2-(2"-aminoethoxy)ethanol salt of dicamba (3,6-dichloro-2-methoxybenzoic acid) decreases the volatility and improves other less advantageous properties of dicamba without detracting from its beneficial herbicidal properties.
Dicamba was described and patented in U.S. Pat. No. 3,013,054 issued Dec. 12, 1961. Since that time it has become a valuable herbicidal product, particularly in the control of weeds in corn. While it is safe to corn plants, it does not harm certain other beneficial crops, such as soybeans. Consequently, care must be taken so that it does not come into contact with such plants.
Dicamba is normally applied as the dimethylamine salt. Unfortunately, this salt is sufficiently volatile that the product applied to a corn crop can come into contact and injure adjacent crops, such as soybeans. This problem cannot be readily solved by the use of additives or other means except very careful application procedures under specified weather conditions. This is often very difficult to accomplish.
It has now been found that by using the previously unknown 2-(2"-aminoethyoxy)ethanol salt of dicamba that the product has a sufficiently low volatility, so as to prevent the product from volatilizing onto undesired valuable crops, and improved solubility without any loss of herbicidal properties. 


2012年5月20日星期日

Toxicokinetics of dicamba (3,6-dichloro-2-methoxy-benzoic acid) and its 3,5-dichloro isomer following intravenous administration to rats


BY: Moheb H. Makary, Joseph C. Street, Raghubir P. Sharma
The distribution and elimination of dicamba (3,6-dichloro-2-methoxy benzoic acid) and another isomer present in dicamba formulation (3,5-dichloro-2-methoxy benzoic acid) were studied in rats following single intravenous injections. Concentrations of dicamba and the isomer were measured at various times in whole blood by EC-GLC after extraction and derivatization with diazomethane. The isomer had different toxicokinetic parameters compared to dicamba. The elimination of dicamba from the blood was rapid with a biological half-life of 0.64 hr (following 100 mg/kg iv dose) while the elimination of the isomer from the blood was much slower with a biological half-life of 16.5 hr (following 20 mg/kg iv dose). However, upon combined dosing of the two compounds the elimination rate constant of dicamba significantly decreased. The isomer had a greater degree of binding to blood serum proteins than dicamba.



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2012年5月18日星期五

About dicamba: Control of common waterhemp (Amaranthus tuberculatus var. rudis) in corn and soybean with sequential herbicide applications


By: Soltani, Nader
Soltani, N., Vyn, J. D. and Sikkema, P. H. 2009. Control of common waterhemp (Amaranthus tuberculatus var. rudis) in corn and soybean with sequential herbicide applications. Can. J. Plant Sci. 89: 127-132. Common waterhemp (Amaranthus tuberculatus) is an aggressive annual broadleaf whose distribution is expected to increase rapidly in agricultural land in eastern Canada. Eight field experiments (four in corn and four in soybean) over a 2-yr period (2005 and 2006) were established on two Ontario farms (near Comber and Petrolia, Ontario) with waterhemp infestations to evaluate the efficacy of various PRE- and POST-emergence herbicides applied alone or in sequence for the control of waterhemp in corn and soybean. There was minimal injury (up to 3.8%) to corn and soybean from the herbicide treatments evaluated. In corn, sequential herbicide programs of isoxaflutole+atrazine PRE fb either dicamba POST, dicamba/diflufenzopyr POST, dicamba/atrazine POST or mesotrione+atrazine POST provided consistent full-season control of waterhemp. Corn yield was reduced 48% when waterhemp was not controlled. Corn yield was equivalent to the weed-free check with the herbicide treatments evaluated. In soybean, PRE or POST herbicides alone provided 41 to 94% control of waterhemp, however, waterhemp control was increased to 90 to 99% with the sequential herbicide programs. Dimethenamid (PRE; 1250 g ha(-1)) followed by glyphosate (POST1; 900 g ha(-1)) followed by glyphosate (POST2; 900 g ha(-1)) controlled waterhemp 99%. Results with waterhemp density and biomass were similar to visible control. Soybean yield was reduced 41% when waterhemp was not controlled. Soybean yield was equivalent to the weed-free check with all the herbicide treatment except dimethenamid PRE, acifluorfen POST1 and fomesafen POST1 where the yield was 30, 19, and 19% lower, respectively.


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2012年5月17日星期四

About dicamba: Studies on herbicides controlled release with crosslinked carboxymethylcellulose


By: Zhuo Renxi; Huang Long ;Zhu Zhifeng
Herbicides 2, 4, 5- T(2, 4, 5 - trichlorophenoxyacetic acid) , 2, 4 - D(2, 4 dichlorophenoxyacetic acid) and dicamba(3, 6 - dichloro - o - anisic acid) were individually encapsulated into crosslinked carboxymethylcellulose (CMC) for the evaluation of their controlled release properties. The release rate of the herbicides into water was greatly influenced by sample form, degree of substitution(DS) of CMC,water solubillity of the herbicides, kind and amount of crosslinking agents and condition for preparing samples. A better controlled release system was achieved by usinggranular sample prepared from the formaldehyde crosslinked CMC with lower DS.



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2012年5月16日星期三

Preparation of polyclonal antibody against dicamba and establishment of indirect competitive ELISA for dicamba detection


By: ZHANG Hua mei,LI Yin lai,PAN Xi ping,JIANG Ai lan,YU Zhen zhen,SHI Man ling
In order to establish the immunoassay method of dicamba(DIC),the diimine carbonization methods were used to synthesize immunogen and coating antigen by dicamba being conjugated to ovalbumin (OVA) and bovine serum albumin (BSA), respectively. The polyclonal antibodies against dicamba were prepared through immunizing New Zealand white rabbits with the immunogen. The enzymelinked immunosorbent assay (ELISA) titer of the antibody was 1∶1.28×105. The percentages of the antibody cross reactivity with other analogues of dicamba (2,3,5-trichlorobenzoic acid, 2,3,6-trichlorobenzoic acid and 2-amino-3,5-dichlorobenzoic acid) were all less than 01%. An indirect competitive ELISA (ciELISA) was developed for dicamba detection, with coating antigen and polyclonal antibody diluted at 1∶8000 and 1∶80000. The lineal range of the assay for detecting dicamba was 1 to 100 ng&#8226;mL-1, with the detection limit (IC20) of 1-779 ng&#8226;mL-1. The regression equation was y = 8.6844 ln x+15.001, R2=0.9944. When dicamba addition value was from 5 to 30 μg&#8226;kg-1 in the recovery tests, the recovery rate of dicamba in corn meal and wheat flour blank samples ranged from 53.08% to 92.37%, and 66.5% to 94.63%, respectively.


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