BY: Weeks, Donald P. Wang, Xiao-zhuo Herman,
Patricia L.
The invention provides isolated and at least partially-purified
dicamba-degrading enzymes, isolated DNA molecules coding for dicamba-degrading
enzymes, DNA constructs coding for dicamba-degrading enzymes, transgenic host
cells comprising DNA coding for dicamba-degrading enzymes, and transgenic plants
and plant parts comprising one or more cells comprising DNA coding for
dicamba-degrading enzymes. Expression of the dicamba-degrading enzymes results
in the production of dicamba-degrading organisms, including dicamba-tolerant
plants. The invention further provides a method of controlling weeds in a field
containing the transgenic dicamba-tolerant plants of the invention and a method
of decontaminating a material containing dicamba comprising applying an
effective amount of a transgenic microorganism or dicamba-degrading enzyme(s) of
the invention to the material. Finally, the invention provides a method of
selecting transformed plants and plant cells based on 
dicamba tolerance and a method of selecting or screening
transformed host cells, intact organisms and parts of organisms based on the
fluorescence of 3,6-dichlorosalicylic acid produced as a result of dicamba
degradationYangzhou pioneer chemical CO.,LTD.
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