About dicamba：Induction and screening of embryonic callus from immature embryo culture of maize inbred line

BY： LI Hai-xia,QIN Wen-juan,TANG Ji-hua,LIU Zong-hua,CHANG Ji-ping,HU Yan-min

Effect of 2,4-D and dicamba on the induction and subculture of callus from maize inbred line was compared with Zheng 58,Zheng 22 and Ye 478 as experimental materials.The results show that the induction effect of dicamba on the calli from the immature embryos of 3 maize inbred lines was better than that of the 2,4-D;the subculture medium with 2 mg L-1 2,4-D adapted to 3 types of genotypes;Zheng 22,more easily induced high quality callus,can be induced directly on induction medium containing 2 mg L-1 2,4-D,then can be cultured on subculture medium containing 2 mg L-1 2,4-D;as for the materials induced callus more difficultly,such as Zheng 58 and Ye 478,they should be induced on induction medium containing 2~3 mg L-1 dicamba,and should be cultured on subculture medium containing 2 mg L-1 2,4-D.This method was conducive to obtain embryonic callus.


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