BY：Jiang-ping REN, Ph D, Xin-guo WANG, Jun
YIN
To establish a highly efficient plantregeneration system for wheat genetic transformation, the effects of three different concentrations of dicamba and two different sugar types on callusinduction and plantregeneration from matureembryocultures were evaluated. Callusinduction and plantregeneration were obtained from matureembryos of two commercial cultivars Zhoumai 18 and Yumai 34 (Triticum aestivum L.) cultured on L3 basal medium. The results showed that the efficiency of matureembryoculture was significantly influenced by the genotypes, sugar types and dicamba concentrations. 4 mg L−1dicamba proved the best effective for inducing embryogenic callus and also gave the highest proportion of plants regenerated across the two cultivars. Substitution of maltose by sucrose significantly improved the plantregeneration efficiency in both cultivars. There was a significant interaction between genotype-by-sugar types, and sugar types-bydicamba concentrations. Overall, Zhoumai 18 gave the highest frequency of plantregeneration (82.65%) when
dicamba concentration was 4.0 mg L−1 and with
sucrose in initial callusinduction. These results will facilitate genetic
transformation work with elite wheat.
To establish a highly efficient plantregeneration system for wheat genetic transformation, the effects of three different concentrations of dicamba and two different sugar types on callusinduction and plantregeneration from matureembryocultures were evaluated. Callusinduction and plantregeneration were obtained from matureembryos of two commercial cultivars Zhoumai 18 and Yumai 34 (Triticum aestivum L.) cultured on L3 basal medium. The results showed that the efficiency of matureembryoculture was significantly influenced by the genotypes, sugar types and dicamba concentrations. 4 mg L−1dicamba proved the best effective for inducing embryogenic callus and also gave the highest proportion of plants regenerated across the two cultivars. Substitution of maltose by sucrose significantly improved the plantregeneration efficiency in both cultivars. There was a significant interaction between genotype-by-sugar types, and sugar types-bydicamba concentrations. Overall, Zhoumai 18 gave the highest frequency of plantregeneration (82.65%) when
dicamba concentration was 4.0 mg L−1 and with
sucrose in initial callusinduction. These results will facilitate genetic
transformation work with elite wheat.Yangzhou pioneer chemical CO.,LTD.
没有评论:
发表评论