In order to establish the immunoassay method of
dicamba（DIC），the diimine carbonization methods were used to synthesize immunogen
and coating antigen by dicamba being conjugated to ovalbumin (OVA) and
bovine serum albumin (BSA), respectively. The polyclonal antibodies against
dicamba were prepared through immunizing New Zealand white rabbits with the
immunogen. The enzymelinked immunosorbent assay (ELISA) titer of the antibody
was 1∶1.28×105. The percentages of the antibody cross reactivity with other
analogues of dicamba (2,3,5-trichlorobenzoic acid, 2,3,6-trichlorobenzoic acid
and 2-amino-3,5-dichlorobenzoic acid) were all less than 01%. An indirect
competitive ELISA (ciELISA) was developed for 
dicamba detection, with coating antigen and polyclonal
antibody diluted at 1∶8000 and 1∶80000. The lineal range of the assay for
detecting dicamba was 1 to 100 ngmL-1, with the detection limit (IC20) of 1-779
ngmL-1. The regression equation was y = 8.6844 ln x+15.001， R2=0.9944. When
dicamba addition value was from 5 to 30 μgkg-1 in the recovery tests, the
recovery rate of dicamba in corn meal and wheat flour blank samples ranged from
53.08% to 92.37%, and 66.5% to 94.63%, respectively.
dicamba detection, with coating antigen and polyclonal
antibody diluted at 1∶8000 and 1∶80000. The lineal range of the assay for
detecting dicamba was 1 to 100 ngmL-1, with the detection limit (IC20) of 1-779
ngmL-1. The regression equation was y = 8.6844 ln x+15.001， R2=0.9944. When
dicamba addition value was from 5 to 30 μgkg-1 in the recovery tests, the
recovery rate of dicamba in corn meal and wheat flour blank samples ranged from
53.08% to 92.37%, and 66.5% to 94.63%, respectively.
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