Preparation of polyclonal antibody against dicamba and establishment of indirect competitive ELISA for dicamba detection

In order to establish the immunoassay method of dicamba（DIC），the diimine carbonization methods were used to synthesize immunogen and coating antigen by dicamba being conjugated to ovalbumin (OVA) and bovine serum albumin (BSA), respectively. The polyclonal antibodies against dicamba were prepared through immunizing New Zealand white rabbits with the immunogen. The enzymelinked immunosorbent assay (ELISA) titer of the antibody was 1∶1.28×105. The percentages of the antibody cross reactivity with other analogues of dicamba (2,3,5-trichlorobenzoic acid, 2,3,6-trichlorobenzoic acid and 2-amino-3,5-dichlorobenzoic acid) were all less than 01%. An indirect competitive ELISA (ciELISA) was developed for dicamba detection, with coating antigen and polyclonal antibody diluted at 1∶8000 and 1∶80000. The lineal range of the assay for detecting dicamba was 1 to 100 ngmL-1, with the detection limit (IC20)　of 1-779 ngmL-1. The regression equation was y = 8.6844 ln x+15.001， R2=0.9944. When dicamba addition　value was from 5 to 30 μgkg-1 in the recovery tests, the recovery rate　of dicamba in corn meal and wheat flour blank samples ranged from 53.08% to 92.37%, and 66.5% to 94.63%, respectively.

Yangzhou pioneer chemical CO.,LTD. 

Pencycuron: a new fungicide against black scurf (Rhizoctonia solani) in potatoes

Pencycuron (Monceren) is a new active ingredient from Bayer AG Leverkusen. Pencycuron is a fungicide with contact effect used as seed dressing in potatoes with a specific effect against black scurf (Rhizoctonia solani). A large number of field trials carried out in several countries in western Europe have proven that pencycuron is setting a new standard for the control of black scurf (Rhizoctonia solani) in potatoes achieving a considerable increase in yield and tuber quality of the crop. Pencycuron has also shown its superiority in comparison to other seed dressings in the market. Furthermore the treatment improves the size and shape of the tubers as shown by the number of tubers having size 35-55 mm with lower Sclerotinia darnage in the harvested crop. Pencycuron was launched in the Danish market in 1993 as Monceren DS 12,5, a dry powder formulation used with a dosage of 200 g per hkg seed potatoes. In 1994 Pencycuron will also be available in Denmark as Monceren FS 250, a liquid formulation used with a dosage of 60 ml per hkg seed potatoes. This formulation is sprayed on the seed tubers while dropping from the potato planter to the soil using special equipment 

Yangzhou pioneer chemical CO.,LTD. 

About dicamba：Induction and screening of embryonic callus from immature embryo culture of maize inbred line

BY： LI Hai-xia,QIN Wen-juan,TANG Ji-hua,LIU Zong-hua,CHANG Ji-ping,HU Yan-min

Effect of 2,4-D and dicamba on the induction and subculture of callus from maize inbred line was compared with Zheng 58,Zheng 22 and Ye 478 as experimental materials.The results show that the induction effect of dicamba on the calli from the immature embryos of 3 maize inbred lines was better than that of the 2,4-D;the subculture medium with 2 mg L-1 2,4-D adapted to 3 types of genotypes;Zheng 22,more easily induced high quality callus,can be induced directly on induction medium containing 2 mg L-1 2,4-D,then can be cultured on subculture medium containing 2 mg L-1 2,4-D;as for the materials induced callus more difficultly,such as Zheng 58 and Ye 478,they should be induced on induction medium containing 2~3 mg L-1 dicamba,and should be cultured on subculture medium containing 2 mg L-1 2,4-D.This method was conducive to obtain embryonic callus.


Yangzhou pioneer chemical CO.,LTD. 

EFSA published review of pesticides risks to bees

Source:EFSA　

EFSA has published a state-of-the-art scientific review of the risks posed by pesticides to honey bees, bumble bees and solitary bees. This major piece of work will support the development of specific guidance for the assessment of possible risks to bees from the use of plant protection products. The guidance will provide up-to-date advice to those involved in the evaluation of plant protection products and their active substances, including industry and public authorities.
The opinion published on the science that will underpin the guidance was requested by the European Commission and responds to growing concerns among MEPs and beekeeper associations about the appropriateness of the current risk assessment scheme. It is also part of EFSA’s coordinated response to the decline in the numbers of honeybees, wild bees and other pollinators in Europe.

Yangzhou pioneer chemical CO.,LTD. 

About pencycuron: Biodegradable solid preparation of a phytopathologicalagent with delayed active substance release

The present invention relates to a method for the preparation of a solid formulation of a slow-release crop protection product, which comprises carrying out a melt extrusion of a composition comprising 0.1 to 80% by weight of at least one fungicidal active ingredient, 0 to 80% by weight of at least one herbicidal, acaricidal, insecticidal or growth-regulatory active ingredient, 3 to 80% by weight of a thermoplastic, water-insoluble polymer　the group of the polylactides, 0 to 80% by weight of at least one thermoplastic water-insoluble polymer, 10 to 80% by weight of at least one mineral filler and 0 to 20% by weight of inorganic or organic additives, and subsequently shaping the extrudate, and to the solid formulations preparable by this method.
1. A method for the preparation of a solid formulation of a slow-release crop protection product, which comprises carrying out a melt extrusion of a composition comprising (a) 0.1 to 80% by weight of at least one fungicidal active ingredient; (b) 0 to 80% by weight of at least one herbicidal, acaricidal, insecticidal or growth-regulatory active ingredient; (c) 3 to 80% by weight of a thermoplastic, water-insoluble polymer　the group of the polylactides; (d) 5 to 80% by weight of at least one polybutylene adipate terephthalate; (e) 10 to 80% by weight of at least one mineral filler; and (f) 0 to 20% by weight of inorganic or organic additives; the total of components (a) to (f) being 100%, and subsequently shaping the extrudate.

2. A method as claimed in claim 1, wherein the composition comprises 5 to 6% by weight of component (d).
3. A method as claimed in claim 1 wherein a fungicidal active ingredient selected　the group consisting of pyroquilon, tricyclazol, isoprothiolane, edifenphos, ferimzone, fludioxinil, pencycuron, flutolanil, mepronil, thifluzamid, iprodione, furametpyr and/or　the class of the strobilurins is used as component (a).



Yangzhou pioneer chemical CO.,LTD. 

About dicamba: Methods and materials for making and using transgenic dicamba-degrading organisms

BY: Weeks, Donald P.　Wang, Xiao-zhuo　 Herman, Patricia L.

The invention provides isolated and at least partially-purified dicamba-degrading enzymes, isolated DNA molecules coding for dicamba-degrading enzymes, DNA constructs coding for dicamba-degrading enzymes, transgenic host cells comprising DNA coding for dicamba-degrading enzymes, and transgenic plants and plant parts comprising one or more cells comprising DNA coding for dicamba-degrading enzymes. Expression of the dicamba-degrading enzymes results in the production of dicamba-degrading organisms, including dicamba-tolerant plants. The invention further provides a method of controlling weeds in a field containing the transgenic dicamba-tolerant plants of the invention and a method of decontaminating a material containing dicamba comprising applying an effective amount of a transgenic microorganism or dicamba-degrading enzyme(s) of the invention to the material. Finally, the invention provides a method of selecting transformed plants and plant cells based on dicamba tolerance and a method of selecting or screening transformed host cells, intact organisms and parts of organisms based on the fluorescence of 3,6-dichlorosalicylic acid produced as a result of dicamba degradation


Yangzhou pioneer chemical CO.,LTD. 

UK: impacts and sustainable use of pesticides in 2011

Source:CRD　

The Pesticides Forum has published its latest annual report ‘Pesticides in the UK – the 2011 report on the impacts and sustainable use of pesticides".
The work of the UK Pesticides Forum in 2011 confirms that the use of pesticides is not adversely impacting on the health of UK citizens or the environment. This is testimony to the effectiveness of both statutory and voluntary controls. However there is scope to reduce risks further without compromising the very real need to control pests, weeds and diseases.
The action plans of the UK Pesticides Strategy identify and help to direct activity resulting in a well-balanced package of statutory and voluntary controls and good advice relating to the responsible use of pesticides. It is important that those who sell, supply, store and use pesticides respect the very necessary legal requirements and engage positively with the innovative voluntary programmes.
This report shows that overall usage of pesticides (measured by weight of active substance) has continued to decline from the peak in 1998. In recent years this drop has largely been as a result of the loss of two key herbicides, isoproturon and trifluralin, and the reduction in maximum application rates of use of others.
These herbicides are all used primarily for the control of black-grass and it remains to be seen how much impact these losses will have on the ability of farmers to control this weed, while minimising the development of resistance to the remaining herbicides used.
One particular important issue the Forum discussed this year was how recent developments in pesticide application technology have helped users apply products more precisely. The UK is at the forefront of these developments. The use of GPS on modern sprayers and developments in spray nozzle technology has helped reduce the risk of drift and overspraying.
We continue to see increases in the number and quality of professional users of pesticides through the provision of well-designed training and continuing professional development programmes. The Forum was pleased to learn of the development of schemes such as Amenity Assured and other initiatives which will help to improve practice in this sector.
The Forum continues to highlight the importance and value of all relevant stakeholders working collaboratively to co-ordinate and provide advice on pesticide use. This will help ensure that society can derive the benefits from responsible pesticide use without being exposed to unacceptable effects. It is important to be innovative, ambitious and to strive to continually reduce risks. The UK public can be assured of the high degree of professionalism exhibited by those who sell, supply, store and use pesticides and that the UK’s robust and innovative range of controls deliver high standards of protection for human health and the environment.

Yangzhou pioneer chemical CO.,LTD.